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PCR Troubleshooting and Optimization: The Essential Guide

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CONTENTS

1. Magic in solution: an indtroduction and brief history of pCR 1
Carl T. Wittwer and Jared S. Farrar

2. qPCR inhibition and amplication of difficult templates 23
Jack M. Gallup

3. Significance of controls and standard curves in PCR 67
Ian Kavanagh, Gerwyn Jones and Saima N. Nayab

4. Obtaining maximum PCR sensitivity and specificity 79
Cameron N. Gundry and Matthew D. Poulson

5. RT-PCR optimization strategies 97
Martina Reiter and Michael W. Pfaffl

6. Real-time PCR instrumetation: an instrument selection guide 119
Sandrina Javorschi-Miller and ivan Delgado Orlic

7. Quantitative PCR data analysis-unlocking the secret to successful results 139

8. The MiQE guidelines uncloaked 151
Greg Shipley

9. PCR applications for epigenetics research 167
Gavin Meredith, Miroslav Dudas, Mark Landers, Vasiliki Anest, Jonathan Wang, Caifu Chen, Peter Jozsi and Christopher Adams

10. High-resolution melting analysis 201
Jhon F, Mackay and Carl T. Wittwer

11. Microfluidic emulsion PCR 217
N. Reginald Beer and jHon L. leamon

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Detail Information

Series Title

-

Call Number

610.28 Ken P

Publisher

UK : Caister Academic Press., 2011

Collation

vii, 235 hal. : ilus. ; 24 cm Index. Hal. 233-235

Language

Indonesia

ISBN/ISSN

978-1-904455-72-1

Classification

610.28 Ken P

Content Type

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Media Type

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Edition

NULL

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