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Ancient Human Tooth DNA Extraction Method: A Rapid Review

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ntroduction: Research on DNA analysis of ancient human teeth is still not optimal due to the lack of the DNA
extraction process. Although teeth are the most resistant part of the body to protect the organic and inorganic
materials, problems in extracting ancient human DNA still occur which results in short DNA molecules which
can complicate the DNA extracting process. This research was conducted to map the success of ancient human
teeth DNA extraction using a variety of substrates that were successfully carried out by previous researchers
Methods: This research was conducted using literature review method through a rapid review approach by
collecting articles through an electronic search engine such as PubMed, EbscoHost, Sicence Direct, Research
Gate, and Cambridge Core that matched the inclusion and exclusion criteria. Results: From 6 articles that
included, the ancient human DNA extraction method with silica-based materials was more often used by
researcher, especially for short DNA base pairs. Conclusion: Extracting ancient human tooth DNA using a silicabased protocol with poor preservation can produced historical age, mtDNA, and microbiota in dental calculus in
fully demineralized samples. Meanwhile, extracting DNA from ancient human teeth using phenol-chloroform can
describe eyes and hair colour of ancient humans.

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Detail Information

Series Title

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Call Number

611 Wiw A

Publisher

FKG UNPAD JATINANGOR : FKG Unpad., 2021

Collation

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Language

English

ISBN/ISSN

160110180028

Classification

611

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

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