MUTAGENISITAS PARAKUAT DIKLORIDA PADA SEL SPERMATID MENCIT SWISS-WEBSTER (Mus musculus) DENGAN UJI MIKRONUKLEUS

Main Authors: Hadiansyah, Cucu, Polianti, Siskasari
Format: Article info application/pdf Journal
Bahasa: ind
Diterbitkan: Biologi Universitas Padjadjaran , 2012
Online Access: http://jurnal.unpad.ac.id/biotika/article/view/153
http://jurnal.unpad.ac.id/biotika/article/view/153/pdf
ctrlnum article-153
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="id-ID">MUTAGENISITAS PARAKUAT DIKLORIDA PADA SEL SPERMATID MENCIT SWISS-WEBSTER (Mus musculus) DENGAN UJI MIKRONUKLEUS</title><creator>Hadiansyah, Cucu</creator><creator>Polianti, Siskasari</creator><description lang="id-ID">Research on the mutagenicity of paraquat to the spermatid of Swiss-Webster mice had been&#xA0; studied&#xA0; by micronucleus assay.&#xA0; The doses of paraquat, which were&#xA0; 17;&#xA0; 25,5;&#xA0; 34 mg/kg bw, physiological saline as negative control, and 40 mg/kg bw of cyclophosphamide as positive control, each administered intraperitoneally at six consecutive times to male Swiss-Webster mice. The spermatid samples taken 16 days later since the first treatment by cell masseration method in trypsin and DNase solution, to formed&#xA0; germ cells suspension.&#xA0; Cells&#xA0; suspension&#xA0; were&#xA0; filtered&#xA0; (mesh&#xA0; no. 325) and centrifuged (100 rpm, 5 minutes) then the pellet were smeared and stained by Giemsa staining. The micronucleus in spermatid (MN-spermatid) were observed in these preparation as a dark blue spherical or ellipsoidal node with size among 1/5 &#x2013; 1/3 to the nucleus diameter and counted per 500 spermatid per animal. The frequencies of MN-spermatid observed after having induced by paraquat was significant (p&lt;0.05) at the dose of 25.5 mg/kg bw and highly significant (p&lt;0.01) at the dose of 34 mg/kg bw to the negative control.&#xA0; The induction of micronucleus by paraquat had a positive correlation&#xA0; to&#xA0; the given doses. On this research, 34 mg/kg bw of paraquat induced 12.0 &#xB1; 2.45&#x2030; were similar (p&lt;0.01) to the induction of 40 mg/kg bw of cyclophosphamide (15.6 &#xB1; 2.00&#x2030;), these indicated that paraquat had a mutagenic effect to the mice spermatid as same as a level of cyclophosphamide mutagenicity.Research on the mutagenicity of paraquat to the spermatid of Swiss-Webster mice had been&#xA0; studied&#xA0; by micronucleus assay.&#xA0; The doses of paraquat, which were&#xA0; 17;&#xA0; 25,5;&#xA0; 34 mg/kg bw, physiological saline as negative control, and 40 mg/kg bw of cyclophosphamide as positive control, each administered intraperitoneally at six consecutive times to male Swiss-Webster mice. The spermatid samples taken 16 days later since the first treatment by cell masseration method in trypsin and DNase solution, to formed&#xA0; germ cells suspension.&#xA0; Cells&#xA0; suspension&#xA0; were&#xA0; filtered&#xA0; (mesh&#xA0; no. 325) and centrifuged (100 rpm, 5 minutes) then the pellet were smeared and stained by Giemsa staining. The micronucleus in spermatid (MN-spermatid) were observed in these preparation as a dark blue spherical or ellipsoidal node with size among 1/5 &#x2013; 1/3 to the nucleus diameter and counted per 500 spermatid per animal. The frequencies of MN-spermatid observed after having induced by paraquat was significant (p&lt;0.05) at the dose of 25.5 mg/kg bw and highly significant (p&lt;0.01) at the dose of 34 mg/kg bw to the negative control.&#xA0; The induction of micronucleus by paraquat had a positive correlation&#xA0; to&#xA0; the given doses. On this research, 34 mg/kg bw of paraquat induced 12.0 &#xB1; 2.45&#x2030; were similar (p&lt;0.01) to the induction of 40 mg/kg bw of cyclophosphamide (15.6 &#xB1; 2.00&#x2030;), these indicated that paraquat had a mutagenic effect to the mice spermatid as same as a level of cyclophosphamide mutagenicity.</description><publisher lang="en-US">Biologi Universitas Padjadjaran</publisher><contributor lang="id-ID"/><date>2012-05-23</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>File:application/pdf</type><identifier>http://jurnal.unpad.ac.id/biotika/article/view/153</identifier><source lang="en-US">Biotika; Vol 1, No 1 (2002): Biotika Juni 2002</source><source lang="id-ID">Biotika; Vol 1, No 1 (2002): Biotika Juni 2002</source><source>1412-4297</source><language>ind</language><relation>http://jurnal.unpad.ac.id/biotika/article/view/153/pdf</relation><rights lang="en-US">Copyright (c) 2016 Biotika</rights><recordID>article-153</recordID></dc>
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format Journal:Article
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author Hadiansyah, Cucu
Polianti, Siskasari
title MUTAGENISITAS PARAKUAT DIKLORIDA PADA SEL SPERMATID MENCIT SWISS-WEBSTER (Mus musculus) DENGAN UJI MIKRONUKLEUS
publisher Biologi Universitas Padjadjaran
publishDate 2012
url http://jurnal.unpad.ac.id/biotika/article/view/153
http://jurnal.unpad.ac.id/biotika/article/view/153/pdf
contents Research on the mutagenicity of paraquat to the spermatid of Swiss-Webster mice had been studied by micronucleus assay. The doses of paraquat, which were 17; 25,5; 34 mg/kg bw, physiological saline as negative control, and 40 mg/kg bw of cyclophosphamide as positive control, each administered intraperitoneally at six consecutive times to male Swiss-Webster mice. The spermatid samples taken 16 days later since the first treatment by cell masseration method in trypsin and DNase solution, to formed germ cells suspension. Cells suspension were filtered (mesh no. 325) and centrifuged (100 rpm, 5 minutes) then the pellet were smeared and stained by Giemsa staining. The micronucleus in spermatid (MN-spermatid) were observed in these preparation as a dark blue spherical or ellipsoidal node with size among 1/5 – 1/3 to the nucleus diameter and counted per 500 spermatid per animal. The frequencies of MN-spermatid observed after having induced by paraquat was significant (p<0.05) at the dose of 25.5 mg/kg bw and highly significant (p<0.01) at the dose of 34 mg/kg bw to the negative control. The induction of micronucleus by paraquat had a positive correlation to the given doses. On this research, 34 mg/kg bw of paraquat induced 12.0 ± 2.45‰ were similar (p<0.01) to the induction of 40 mg/kg bw of cyclophosphamide (15.6 ± 2.00‰), these indicated that paraquat had a mutagenic effect to the mice spermatid as same as a level of cyclophosphamide mutagenicity.Research on the mutagenicity of paraquat to the spermatid of Swiss-Webster mice had been studied by micronucleus assay. The doses of paraquat, which were 17; 25,5; 34 mg/kg bw, physiological saline as negative control, and 40 mg/kg bw of cyclophosphamide as positive control, each administered intraperitoneally at six consecutive times to male Swiss-Webster mice. The spermatid samples taken 16 days later since the first treatment by cell masseration method in trypsin and DNase solution, to formed germ cells suspension. Cells suspension were filtered (mesh no. 325) and centrifuged (100 rpm, 5 minutes) then the pellet were smeared and stained by Giemsa staining. The micronucleus in spermatid (MN-spermatid) were observed in these preparation as a dark blue spherical or ellipsoidal node with size among 1/5 – 1/3 to the nucleus diameter and counted per 500 spermatid per animal. The frequencies of MN-spermatid observed after having induced by paraquat was significant (p<0.05) at the dose of 25.5 mg/kg bw and highly significant (p<0.01) at the dose of 34 mg/kg bw to the negative control. The induction of micronucleus by paraquat had a positive correlation to the given doses. On this research, 34 mg/kg bw of paraquat induced 12.0 ± 2.45‰ were similar (p<0.01) to the induction of 40 mg/kg bw of cyclophosphamide (15.6 ± 2.00‰), these indicated that paraquat had a mutagenic effect to the mice spermatid as same as a level of cyclophosphamide mutagenicity.
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